COVID-19 Priority Research Paper Volume 13, Issue 18 pp 21838—21854

SARS-CoV-2 causes senescence in human cells and exacerbates the senescence-associated secretory phenotype through TLR-3

SARS-CoV-2 amplifies the SASP of senescent preadipocytes without infecting them. (A) Senescent and non-senescent preadipocytes were exposed to SARS-CoV-2 for the indicated times and assayed for infection by qPCR. (B) Senescent preadipocytes were treated with SARS-CoV-2 and the SASP was assayed 82 hrs. later by qPCR. Data are expressed as a function of untreated senescent cells; mean +/- SEM, repeated 1-way ANOVA and post hoc comparison pairwise Tukey’s HSD. All other significant p values are listed in Supplementary Table 1.

Figure 4. SARS-CoV-2 amplifies the SASP of senescent preadipocytes without infecting them. (A) Senescent and non-senescent preadipocytes were exposed to SARS-CoV-2 for the indicated times and assayed for infection by qPCR. (B) Senescent preadipocytes were treated with SARS-CoV-2 and the SASP was assayed 82 hrs. later by qPCR. Data are expressed as a function of untreated senescent cells; mean +/- SEM, repeated 1-way ANOVA and post hoc comparison pairwise Tukey’s HSD. All other significant p values are listed in Supplementary Table 1.