Research Paper Volume 13, Issue 18 pp 22528—22543

Inhibition of FSTL3 abates the proliferation and metastasis of renal cell carcinoma via the GSK-3β/β-catenin signaling pathway


Figure 2. FSTL3 overexpression facilitated RCC proliferation and metastasis and cramped cell apoptosis. A498 and ACHN cells were transiently transfected along with FSTL3 overexpression plasmids. (AC) qRT-PCR (A), Western Blot (B), and ELISA (C) were done for FSTL3 detection; (D, E) BrdU (D) and CCK8 (E) were implemented to examine cell proliferation; (F) Flow cytometry measured cell apoptosis; (G) Transwell evaluated cell invasion; (H) Western Blot assessed E-cadherin, Twist, and Slug expression in RCC cells. **P<0.01, ***P<0.001 (vs. the NC group). N=5.