Research Paper Volume 13, Issue 18 pp 22528—22543

Inhibition of FSTL3 abates the proliferation and metastasis of renal cell carcinoma via the GSK-3β/β-catenin signaling pathway

FSTL3 knockdown impeded RCC proliferation and metastasis and accelerated cell apoptosis. A498 and ACHN cells were transiently transfected along with si-FSTL3. (A–C) qRT-PCR (A), Western Blot (B), and ELISA (C) were taken for FSTL3 detection; (D, E) BrdU (D) and CCK8 (E) analyzed cell proliferation; (F) Flow cytometry examined cell apoptosis; (G) Transwell assessed cell invasion; (H) Western Blot evaluated E-cadherin, Twist, and Slug expression in RCC cells. *PPP

Figure 3. FSTL3 knockdown impeded RCC proliferation and metastasis and accelerated cell apoptosis. A498 and ACHN cells were transiently transfected along with si-FSTL3. (AC) qRT-PCR (A), Western Blot (B), and ELISA (C) were taken for FSTL3 detection; (D, E) BrdU (D) and CCK8 (E) analyzed cell proliferation; (F) Flow cytometry examined cell apoptosis; (G) Transwell assessed cell invasion; (H) Western Blot evaluated E-cadherin, Twist, and Slug expression in RCC cells. *P<0.05, **P<0.01, ***P<0.001 (vs. the si-NC group). N=5.