Figure 4. FSTL3 knockdown suppressed RCC proliferation and metastasis and boosted cell apoptosis. (A) The conditioned medium (CM) of RCC cells with FSLT3 overexpression or down-regulation was collected. RCC cells were with the CM. A498 and ACHN cells were transiently transfected along with si-FSTL3. (B, C) BrdU (B) and CCK8 (C) monitored cell proliferation. (D, E) Flow cytometry examined cell apoptosis; (F) Transwell assessed cell invasion; (G) Western Blot evaluated E-cadherin, Twist, and Slug profile in RCC cells. *P<0.05, **P<0.01, ***P<0.001 (vs. the Blank group). &P<0.05, &&P<0.01, &&&P<0.001 (vs. the CMNC or CMsi-NC group). N=5.