Figure 7. β-catenin inhibition attenuated FSTL3-induced RCC progression. FSTL3 overexpression plasmids were transfected into ACHN cells, and XAV939 (1 μM) or si-β-catenin was added for intervention post FSTL3 overexpression. (A–C) qRT-PCR (A), western blot (B), and ELISA (C) were deployed for FSTL3 detection; (D–G) CCK8 (D), BrdU (E), Flow cytometry (F), and Transwell (G) monitored cell proliferation, apoptosis, and invasion; (H–J) The protein expressions of the EMT, GSK-3β/β-catenin, and BMP1/SMAD pathways were confirmed via Western Blot. *P<0.05, **P<0.01, ***P<0.001. N=5.