Figure 2. The effect of Glaucocalyxin B on the expression of CD86 and P65. (A–B) IF staining of CD86 and P65 showed that the levels of CD86 and P65 were significantly up-regulated in M1 SMG, which were higher than DMSO. Gla B could inhibit the expression of P65 and CD86. (C–D) Western-Blot (n = 3), the levels of P65 and p-P65 were significantly up-regulated in M1 SMM, which were higher than DMSO. Gla B could inhibit the expression of p-P65 and P65. Comparison with DMSO, *P < 0.05; comparison with L/I, #P < 0.05.