Research Paper Volume 13, Issue 18 pp 22544—22555

Glaucocalyxin B inhibits cartilage inflammatory injury in rheumatoid arthritis by regulating M1 polarization of synovial macrophages through NF-κB pathway

The effect of Glaucocalyxin B on the expression of CD86 and P65. (A–B) IF staining of CD86 and P65 showed that the levels of CD86 and P65 were significantly up-regulated in M1 SMG, which were higher than DMSO. Gla B could inhibit the expression of P65 and CD86. (C–D) Western-Blot (n = 3), the levels of P65 and p-P65 were significantly up-regulated in M1 SMM, which were higher than DMSO. Gla B could inhibit the expression of p-P65 and P65. Comparison with DMSO, *P #P

Figure 2. The effect of Glaucocalyxin B on the expression of CD86 and P65. (AB) IF staining of CD86 and P65 showed that the levels of CD86 and P65 were significantly up-regulated in M1 SMG, which were higher than DMSO. Gla B could inhibit the expression of P65 and CD86. (CD) Western-Blot (n = 3), the levels of P65 and p-P65 were significantly up-regulated in M1 SMM, which were higher than DMSO. Gla B could inhibit the expression of p-P65 and P65. Comparison with DMSO, *P < 0.05; comparison with L/I, #P < 0.05.