Figure 6. The effect of Gla B on the expression of M1 type cells and P65 in bone tissue. (A) Detection of CD86 and P65 revealed that the levels of CD86 and P65 were relatively low in Control group, without infiltration of M1 type cells. The expression of CD86 and P65 was significantly up-regulated in RA, with obvious M1 polarization. While Gla B could inhibit the expression of C86 and P65. (B–E) Detection of M1 type cell markers. The levels of TNF-α, IL-1β, IL-6 and iNOS were significantly higher in RA than those in Control group. Gla B could inhibit the expression of TNF-α, IL-1β, and IL-6 and iNOS, which was significantly decreased compared with RA. Comparison with Control, *P < 0.05; comparison with RA, #P < 0.05.