Figure 7. Interaction of PAK4 with PPARγ to regulate Nox1 expression. Robust interaction between PAK4 and PPARγ was confirmed by IP (A); PAK4 and PPARγ expressions in macrophage by LPS+ATP (B); PPARγ binding with the Nox1 promoter in macrophage by LPS+ATP (C); PAK4, PPARγ and Nox1 protein expressions in macrophage by LPS+ATP+4-CMTB (D, E). Control, control group; LPS+ATP+4-CMTB, macrophage by treated with LPS+ATP+4-CMTB. ##p<0.01 compared with control group; **p<0.01 compared with down-regulation of GPR43.