Research Paper Volume 13, Issue 22 pp 24560—24579

Circular RNA CELF1 drives immunosuppression and anti-PD1 therapy resistance in non-small cell lung cancer via the miR-491-5p/EGFR axis


Figure 1. The high circ_CELF1 expression in NSCLC tissues and prognostic significance. (A) The heatmap shows circRNAs derived from the CELF1 gene in NSCLC tissues compared with those in adjacent normal tissues analyzed by qRT-PCR. (B) The differential expression of circ_CELF1 in NSCLC tissues and adjacent normal tissues NSCLC patients was determined by qRT-PCR. n = 37, *P < 0.05. (C) The expression level of circ_CELF1 in NSCLC cell lines (PG49, H2170, A549, H1975) was detected by qRT-PCR assay, and BEAS-2B was indicated as control. n = 5, *P < 0.05. (D and E) Survival (D) and recurrence (E) analysis of circ_CELF1 high and low patients in 30 NSCLC patients. (F) ROC curve analysis was performed to evaluate the diagnostic value of circ_CELF1 in NSCLC by using 37 primary NSCLC tissues.