Figure 5. KIF4A accelerated cell cycle and proliferation of ESCC cells. KYSE150 and TE1 ESCC cells were transfected with shRNAs specifically targeting KIF4A or scrambled shRNA control (NC). The effective knockdown of KIF4A in these ESCC cells was confirmed by WB (A) and RT-qPCR (B). Cell proliferation of these KIF4A knockdown and NC cells were detected by CCK8 assay (C). Colony formation was detected by single-cell clone assay (scale bar 50 um) (D). Representative images of cell cycle distributions of KYSE-150 and TE-1 cells transfected with control or KIF4A shRNAs for 48 h were determined by flow cytometry (E). Results are representative of three independent experiments performed in triplicate. The data are presented as the means ± SD. Statistically significant difference: *P < 0.05, **P < 0.01, ***P < 0.001, n = 3.