KIF4A facilitated invasion and migration of ESCC cells. Transwell assay was performed to compare migration and invasion between cells treated with shRNAs and NC. The representative images of migrated and invasive cells are shown (A–B) (scale bar 50 um). The presented figures are representative data from at least three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 for statistical analysis of the indicated groups.