KIF4A promoted PI3K/AKT and MAPK pathways and EMT in ESCC cells. Comparing shRNA KIF4A with their respective control cells (NC) were seen in relative expression of P-AKT, AKT, P-ERK1/2, ERK1/2, P-JNK, JNK, P-P38, P38, and GAPDH was taken as control by WB. In addition, E-cadherin, N-cadherin, and vimentin were also detected by WB. Data represent mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, n = 3.