Figure 3. ZnO NPs affect gene expression at the translational level. (A) Schematic of the experimental design. Cells were treated with 5 μg/mL of ZnO NPs. (B) Volcano plots of genes with differential ribosome occupancy in control and ZnO NP-treated cells. (C) Correlation of RNA-seq data and Ribo-seq data between control and ZnO NPs-treated group. (D) Scatterplot of mRNA expression and ribosome profiling data from control and ZnO NPs-treated cells. The number of up-regulated and down-regulated genes at mRNA level and translational level was indicated. (E) Heat map diagram of differentially expressed genes. (F) KEGG pathway of translationally down-regulated genes. (G) KEGG pathway of translationally up-regulated genes.