Research Paper Volume 13, Issue 21 pp 24071—24085

LncRNA MIR31HG is induced by tocilizumab and ameliorates rheumatoid arthritis fibroblast-like synoviocyte-mediated inflammation via miR-214-PTEN-AKT signaling pathway

Anti-inflammatory effects of tocilizumab on RA-FLS-mediated inflammation. (A) The sorting strategy for isolating RA-FLS from joint synovium of RA patients. (B, C) The effects of tocilizumab on the proliferation and migration abilities of RA-FLS (n=3). (D, E) The effects of tocilizumab on various cytokines and MMPs of RA-FLS (n=3). (F, G) LncRNA-seq and miRNA-seq were performed using tocilizumab-treated RA-FLS. (H) Strategy for selecting potential tocilizumab lncRNA and miRNA targets in RA-FLS for downstream experimental validation. Data represent the mean ± SEM; *p t-test.

Figure 1. Anti-inflammatory effects of tocilizumab on RA-FLS-mediated inflammation. (A) The sorting strategy for isolating RA-FLS from joint synovium of RA patients. (B, C) The effects of tocilizumab on the proliferation and migration abilities of RA-FLS (n=3). (D, E) The effects of tocilizumab on various cytokines and MMPs of RA-FLS (n=3). (F, G) LncRNA-seq and miRNA-seq were performed using tocilizumab-treated RA-FLS. (H) Strategy for selecting potential tocilizumab lncRNA and miRNA targets in RA-FLS for downstream experimental validation. Data represent the mean ± SEM; *p < 0.05, as determined by the Student’s t-test.