Curcumol inhibited the self-renewal ability of glioma cells. (A) CD133, Nanog, and DAPI staining were shown by immunostaining in neurosphere from glioma stem-like cells with or without 40 μg/mL curcumol. Scale bar = 200 μm. (B) Neurosphere formation capacity of glioma stem-like cells was determined. Curcumol treatment reduced the neurosphere formation of glioma stem-like cells, Scale bar = 150 μm. (C) The percentage of CD133+ cells was analyzed by FCM. (D) The expressions of stem cell marker genes in glioma cells were detected by western blotting analysis. *p < 0.05, **p < 0.01 when compared with the untreated control group.