Figure 5. Over-expression of FoxD2-As1 reduced the anti-proliferation, anti-metastasis, and pro-apoptosis effects of curcumol in glioma cells. (A) qRT-PCR was performed to determine FOXD2-As1 expression in glioma cells of each group. pcDNA3.1-FOXD2-As1 transfection markedly increased the expression of FOXD2-As1 in glioma cells while the upregulated FOXD2-As1 expression was inhibited by curcumol treatment. (B) MTT assay was performed to determine the viability of each group cells after treated with curcumol for 48 h. (C) Transwell migration and (D) invasion assays were performed to examine the metastasis of each group cells after treated with curcumol for 48 h. (E) Annexin V-FITC/PI apoptosis assay was performed to examine the apoptotic rate of each group cells after treated with curcumol for 48 h. Data were represented as means ± SD from at least of three independent experiments. *p < 0.05, **p < 0.01.