Figure 6. Over-expression of FoxD2-As1 abolished the inhibitory effect of curcumol on the self-renewal ability of glioma cells. (A) Immunostaining showed the expression of CD133 and Nanog on the membrane of glioma stem-like cells in each group, Scale bar = 200 μm. (B) Neurosphere formation assay showed that FoxD2-As1 upregulation promoted curcumol reduced neurosphere formation capacity of glioma cells in stem-like conditions, Scale bar = 150 μm. (C) Flow cytometry analysis showed that curcumol reduced CD133+ cells were increased by upregulation of FoxD2-As1. (D) Western blotting analysis showed that forced expression of FoxD2-As1 reversed curcumol-mediated decrease of CD133, Nestin, Nanog, and SOX-2 protein expression. *p < 0.05, **p < 0.01.