Research Paper Volume 13, Issue 21 pp 24101—24116

Curcumol inhibits malignant biological behaviors and TMZ-resistance in glioma cells by inhibiting long noncoding RNA FOXD2-As1-promoted EZH2 activation

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Figure 7.

Over-expression of FoxD2-As1 abolished the effect of curcumol on TMZ-resistance of glioma cells. (A) MTT assay showed that FoxD2-As1 overexpression increased the cell viability compared to control and empty vector transfection groups. (B) Colony formation assay showed that the clonogenicity of TMZ-resistant glioma cells reduced by curcumol and TMZ treatment was reversed by FoxD2-As1 overexpression. (C) FoxD2-As1 overexpression significantly reduced the ratio of methylated MGMT promoter DNA and increased MGMT protein level in TMZ-resistant glioma cells. *P < 0.05, **P < 0.01. FoxD2-As1 overexpression VS. control group; #P < 0.05, ##P < 0.01. FoxD2-As1 overexpression VS. empty vector group.