Research Paper Volume 13, Issue 21 pp 24101—24116

Curcumol inhibits malignant biological behaviors and TMZ-resistance in glioma cells by inhibiting long noncoding RNA FOXD2-As1-promoted EZH2 activation

Curcumol inhibited the activation of EZH2 through the downregulation of FoxD2-As1. ChIP (A) and DNA pull down (B) assay showed the binding of EZH2 and H3K27me3 to the promoters of p21, EphB3, and CDKN1B. (C) curcumol inhibited the mRNA expression level of p21, EphB3, and CDKN1B in U251 and A172 cells. (D) curcumol inhibited the expression of EZH2 in U251 and A172 cells. *P **P #P ##P

Figure 8. Curcumol inhibited the activation of EZH2 through the downregulation of FoxD2-As1. ChIP (A) and DNA pull down (B) assay showed the binding of EZH2 and H3K27me3 to the promoters of p21, EphB3, and CDKN1B. (C) curcumol inhibited the mRNA expression level of p21, EphB3, and CDKN1B in U251 and A172 cells. (D) curcumol inhibited the expression of EZH2 in U251 and A172 cells. *P < 0.05, **P < 0.01. FoxD2-As1 overexpression VS. control group; #P < 0.05, ##P < 0.01. FoxD2-As1 overexpression VS. empty vector group.