Research Paper Volume 13, Issue 21 pp 24171—24191

LncRNA OTUD6B-AS1 promotes paclitaxel resistance in triple negative breast cancer by regulation of miR-26a-5p/MTDH pathway-mediated autophagy and genomic instability

Identification of OTUD6B-AS1-miR-26a-5p-MTDH signaling pathway. (A) miRDB, miRTarBase, and TargetScan databases are used to identify 133 miR-26a-5p targeted genes. (B) 541 lncRNAs are collected from The LncBase 2.0 which interacts with miR-26a-5p, among which 144 lncRNAs are found in 13998 breast cancer-associated lncRNAs; 5 prognosis-related lncRNAs is identified. (C) The prognosis risk of 5 lncRNAs in breast cancer. (D) K-M analysis of 5 lncRNAs, data from TCGA. (E) The top5 genes with the most strength correlation of 5 lncRNAs. (F) WB assay to detect the level of MTDH in HCC1937 after different treatments. (G) RT-qPCR to detect the level of miR-26a-5p. (H) Luciferase to detect the interaction between miR-26a-5p and OTUD6B-AS1.

Figure 4. Identification of OTUD6B-AS1-miR-26a-5p-MTDH signaling pathway. (A) miRDB, miRTarBase, and TargetScan databases are used to identify 133 miR-26a-5p targeted genes. (B) 541 lncRNAs are collected from The LncBase 2.0 which interacts with miR-26a-5p, among which 144 lncRNAs are found in 13998 breast cancer-associated lncRNAs; 5 prognosis-related lncRNAs is identified. (C) The prognosis risk of 5 lncRNAs in breast cancer. (D) K-M analysis of 5 lncRNAs, data from TCGA. (E) The top5 genes with the most strength correlation of 5 lncRNAs. (F) WB assay to detect the level of MTDH in HCC1937 after different treatments. (G) RT-qPCR to detect the level of miR-26a-5p. (H) Luciferase to detect the interaction between miR-26a-5p and OTUD6B-AS1.