Construction and external validation of a 5-gene random forest model to diagnose non-obstructive azoospermia based on the single-cell RNA sequencing of testicular tissue

Ranran Zhou; Xianyuan Lv; Tianle Chen; Qi Chen; Hu Tian; Cheng Yang; Wenbin Guo; Cundong Liu

doi:doi:10.18632/aging.203675

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Research Paper Volume 13, Issue 21 pp 24219—24235

Construction and external validation of a 5-gene random forest model to diagnose non-obstructive azoospermia based on the single-cell RNA sequencing of testicular tissue

Figure 8. The validation in human seminal plasma samples. (A–E) The expression level of CCT8 (A), CDC6 (B), PSMD1 (C), RPL36A (D), and RPS4X (E) in human seminal plasma from 20 OA and 20 NOA patients. (F, G) The ROC (F) and confusion matrix (G) displayed that the RF model was a promising classifier in the collected human samples. NOA, non-obstructive azoospermia; OA, obstructive azoospermia. *, P < 0.05; **, P < 0.01; ***, P < 0.001.