Research Paper Volume 13, Issue 21 pp 23953—23980

UBB+1 reduces amyloid-β cytotoxicity by activation of autophagy in yeast

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Figure 6.

Low UBB+1 expression reduces Aβ42 levels in the humanized yeast AD model. (A) Immunostaining analysis of Aβ42 localization and expression using the 6E10 Aβ specific antibody. Nuclei were stained blue by DAPI. Scale bar = 5 μm. (B) Western blot analysis of Aβ42 expression in unboiled cell lysates with 6E10 antibody. GAPDH was used as the loading control. (C) Relative Aβ42 band intensity was normalized to GAPDH and compared to the untreated Aβ42 strain. Results are reported as mean ± SD of three independent experiments. *p < 0.05.