Research Paper Volume 13, Issue 21 pp 24339—24348

Deletion of miR-15a inhibited glioma development via targeting Smad7 and inhibiting EMT pathway


Figure 2. Silencing of miR-15a inhibited the malignancy of SHG139 cells. (A) SHG139 cells were transfected with miR-15a or its negative control (miR-NC), and qRT-PCR used to test the transfection efficiency. (B) AMO-15a or its negative control (AMO-NC) were transfected into SHG139 cells, and miR-15a level in SHG139 cells were detected. (C) Wound healing assay was used to detect cell migration. Scale bar, 200 μm. (DF) Transwell assay was performed to identify the invasion of SHG139 cells. Scale bar, 50 μm. Data were expressed as mean ± SD.*P < 0.05.