Figure 5. Suppressed the expression of miR-15a prevented tumorigenesis in vivo. The SHG139 cell lines stably expressing AMO-15a were inoculated into nude mice to establish glioma mouse model. (A) Representative images of separated tumors from nude mice after 30 days of inoculation. The relative tumor volume (B) and weight (C) was calculated. (D) The expression of miR-15a in tumors was tested. (E) The protein expression of Smad7 and EMT relative markers (Vimentin, N-cadherin and E-cadherin) was tested by western blot. (F) The Ki67 expression was test by IHC. Scale bar, 50 μm. Data were expressed as mean ± SD.*P < 0.05.