Research Paper Volume 13, Issue 22 pp 24580—24604

BMPR2 promoter methylation and its expression in valvular heart disease complicated with pulmonary artery hypertension

BMPR2 was downregulated in human PAH patients as well as in the PAH model mice. (A–C) Western blot analysis of human patient samples. (A) Western blot analysis was carried out to examine BMPR2 and BMP4 protein levels in the heart valve tissues samples derived from the human PAH patients after surgery with mild (30 mmHg ≤ PASP Figure 6B for BMPR2 and Figure 6C for BMP4. Mild PAH, patients with low PASP. Moderate/severe PAH, patients with moderate to severe PASP. (D–F) Western blot analysis of mouse PAH samples. (D) Western blot analysis was carried out to examine BMPR2 and BMP4 protein levels in the heart tissues samples derived from the PAH model mice. The band intensities of BMPR2 and BMP4 in three control (C1, C2, C3) and three PAH model mice (P1, P2, P3) were normalized to those of GAPDH on the western blots based with the ImageJ analysis. The numbers are shown for each band of BMPR2 and BMP4 in the PAH model mice. These normalized protein levels are shown in Figure 6E for BMPR2 and Figure 6F for BMP4. Statistical analysis: *P **P

Figure 6. BMPR2 was downregulated in human PAH patients as well as in the PAH model mice. (AC) Western blot analysis of human patient samples. (A) Western blot analysis was carried out to examine BMPR2 and BMP4 protein levels in the heart valve tissues samples derived from the human PAH patients after surgery with mild (30 mmHg ≤ PASP < 55 mmHg) and moderate to severe (PASP ≥ 55 mmHg) pulmonary hypertension. The band intensities of BMPR2 and BMP4 in four low PAH patients (#19, #22, #33, #34) and four moderate/severe PAH patients (#29, #39, #47, #48) were normalized to those of GAPDH on the western blots based with the ImageJ analysis. The numbers are shown for each band of BMPR2 and BMP4 in each patient sample. These normalized protein levels are shown in Figure 6B for BMPR2 and Figure 6C for BMP4. Mild PAH, patients with low PASP. Moderate/severe PAH, patients with moderate to severe PASP. (D–F) Western blot analysis of mouse PAH samples. (D) Western blot analysis was carried out to examine BMPR2 and BMP4 protein levels in the heart tissues samples derived from the PAH model mice. The band intensities of BMPR2 and BMP4 in three control (C1, C2, C3) and three PAH model mice (P1, P2, P3) were normalized to those of GAPDH on the western blots based with the ImageJ analysis. The numbers are shown for each band of BMPR2 and BMP4 in the PAH model mice. These normalized protein levels are shown in Figure 6E for BMPR2 and Figure 6F for BMP4. Statistical analysis: *P < 0.05. **P < 0.01.