Research Paper Volume 13, Issue 22 pp 24580—24604

BMPR2 promoter methylation and its expression in valvular heart disease complicated with pulmonary artery hypertension

Apoptosis was increased in the PAH model mice. TUNEL staining was used to examine apoptotic cells on the heart sections of the control and PAH model mice. (A) the image of the DAPI-stained (blue staining) heart section of the control mice. (B) the image of the TUNEL-stained (green staining) heart section of the control mice. Arrow, TUNEL-stained apoptotic cell. (C) the merged image of DAPI-stained (blue staining) and TUNEL-stained (green staining) heart section of the control mice. Arrow, TUNEL-stained apoptotic cell. (D) the image of the DAPI-stained (blue staining) heart section of the PAH mice. (E) the image of the TUNEL-stained (green staining) heart section of the PAH mice. Arrow, TUNEL-stained apoptotic cell. (F) the merged image of DAPI-stained (blue staining) and TUNEL-stained (green staining) heart section of the PAH mice. Arrow, TUNEL-stained apoptotic cell.

Figure 7. Apoptosis was increased in the PAH model mice. TUNEL staining was used to examine apoptotic cells on the heart sections of the control and PAH model mice. (A) the image of the DAPI-stained (blue staining) heart section of the control mice. (B) the image of the TUNEL-stained (green staining) heart section of the control mice. Arrow, TUNEL-stained apoptotic cell. (C) the merged image of DAPI-stained (blue staining) and TUNEL-stained (green staining) heart section of the control mice. Arrow, TUNEL-stained apoptotic cell. (D) the image of the DAPI-stained (blue staining) heart section of the PAH mice. (E) the image of the TUNEL-stained (green staining) heart section of the PAH mice. Arrow, TUNEL-stained apoptotic cell. (F) the merged image of DAPI-stained (blue staining) and TUNEL-stained (green staining) heart section of the PAH mice. Arrow, TUNEL-stained apoptotic cell.