The influences of RIP3 inhibitor and JNK inhibitor on the levels of RIP3 and p-JNK in the lumbar spinal cords of CCI rats. (A) The levels of RIP3, p-JNK, JNK, c-Fos, and GAPDH in the lumbar spinal cords of rats on 1 day before CCI and 3, 7, and 14 days after CCI. (B) The ratio of RIP3, c-Fos to GAPDH and the ratio of p-JNK to JNK were analyzed. (C) The expression of RIP3 in lumbar spinal cord by immunofluorescence on 14 days after CCI. (D) Quantification of immunofluorescence area of (C) by Image J. Data are shown as mean SD (n = 8). *P < 0.05, in contrast with the control group, #P < 0.05, in contrast with the CCI + NaCl group.