Research Paper Volume 13, Issue 22 pp 24605—24620

Knockdown of transient receptor potential melastatin 2 reduces renal fibrosis and inflammation by blocking transforming growth factor-β1-activated JNK1 activation in diabetic mice


Figure 3. Silencing TRPM2 attenuated the renal function of diabetic mice. (A) Body weight of mice was documented from week 0 to 24 after the HFD/STZ-induced diabetes model was established. (B) Blood glucose levels in the four treatment groups were measured in the indicated weeks. (C, D) Protein assay of TRPM2 expression in renal specimens from AAV2/2-NC and AAV2/2-shTRPM2 treated mice. (E) IF double-staining analyses of TRPM2 and APQ1 in the renal tissues of mice in the 24th week in the four treatment groups. (F, G) The levels of blood creatinine and BUN were tested before the mice were sacrificed in the four treatment groups. The data are shown as the mean ± SD, n = 6 per group; *p < 0.05, **p < 0.01 and ***p < 0.001. For (A, B), *p < 0.05 and ***p < 0.001 indicate the AAV2/2-NC + STZ group versus the AAV2/2-NC group, #p < 0.05 and ###p < 0.001 indicate the AAV2/2-shTRPM2 + STZ group versus the AAV2/2-shTRPM2 group.