Research Paper Volume 13, Issue 22 pp 24605—24620

Knockdown of transient receptor potential melastatin 2 reduces renal fibrosis and inflammation by blocking transforming growth factor-β1-activated JNK1 activation in diabetic mice


Figure 4. TRPM2 silencing attenuated renal damage in mice with HFD/STZ-induced diabetes. (A) HE and PAS staining of mouse kidney specimens in the four treatment groups in the 24th week (▼indicates renal glomeruli, ˃ indicates renal tubules). (BD) Fibrosis was determined by Masson and Sirius red staining in the four treatment groups (▼ indicates renal glomeruli, ˃ indicates renal tubules, ↓ indicates renal interstitials). (E, F) Protein assay was implemented to evaluate the protein levels of TGF-β1, CTGF, α-SMA, FN, Collagen I and Collagen III in the renal specimens from the four treatment groups. (G, H) Protein assays were conducted to evaluate the protein levels of P-Smad2 and Smad2 in the renal specimens of the four treatment groups. The data are shown as the mean ± SD, n = 6 per group; *p < 0.05, **p < 0.01 and ***p < 0.001.