Research Paper Volume 13, Issue 22 pp 24605—24620

Knockdown of transient receptor potential melastatin 2 reduces renal fibrosis and inflammation by blocking transforming growth factor-β1-activated JNK1 activation in diabetic mice


Figure 5. Silencing TRPM2 alleviated inflammation in the kidneys of mice with HFD/STZ-induced diabetes. (A) qRT-PCR and (B) ELISA analysis of the IL-1β, IL-6, IFN-α, TNF-α and MCP-1 levels in the kidney tissues of mice in the four treatment groups in the 24th week. (C, D) Western blotting assays were conducted to evaluate the protein levels of p-JNK1, p-IKKα and p-NF-κB in the renal specimens of the four treatment groups. (E, F) Western blotting of the NF-κB levels in the nuclei of the renal specimens in the four treatment groups. The data are shown as the mean ± SD, n = 6 per group; *p < 0.05, **p < 0.01 and ***p < 0.001.