Research Paper Volume 13, Issue 21 pp 24449—24463

Small interfering RNA-induced silencing lncRNA PVT1 inhibits atherosclerosis via inactivating the MAPK/NF-κB pathway

Silencing PVT1 reduces AS damage in ApoE-/- mice. ApoE-/- mice were fed with high-fat diet for 12 weeks. (A) Relative PVT1 expression in mice detected by qRT-PCR; (B) Levels of TG, TC, HDL, and LDL detected by ELISA; (C) Lipid accumulation on the aortic wall detected by oil red O staining; (D) mRNA levels of MMP-2, MMP-9, CRP, and TIMP-1 detected by qRT-PCR; (E) Protein levels of MMP-2, MMP-9, CRP and TIMP-1 detected by Western blot; (F) Levels of IL-6, IL-1β, and TNF-α detected by ELISA; (G) CD68 positive cells detected by immunohistochemistry. N = 6. Data were presented as mean ± standard deviation. Comparisons among multi-groups were analyzed using one-way ANOVA, followed by Tukey’s multiple comparisons test. * p  0.05, ** p  0.01. PVT1, plasmacytoma variant translocation 1; AS, atherosclerosis; ApoE-/-, apolipoprotein E knockout; TG, total triglyceride; TC, total cholesterol; HDL, high density lipoprotein; LDL, low density lipoprotein; MMP, matrix metalloproteinase; TIMP-1, tissue inhibitor of metalloproteinase-1; CRP, C-reactive protein; IL, interleukin; TNF-α, tumor necrosis factor-alpha.

Figure 2. Silencing PVT1 reduces AS damage in ApoE-/- mice. ApoE-/- mice were fed with high-fat diet for 12 weeks. (A) Relative PVT1 expression in mice detected by qRT-PCR; (B) Levels of TG, TC, HDL, and LDL detected by ELISA; (C) Lipid accumulation on the aortic wall detected by oil red O staining; (D) mRNA levels of MMP-2, MMP-9, CRP, and TIMP-1 detected by qRT-PCR; (E) Protein levels of MMP-2, MMP-9, CRP and TIMP-1 detected by Western blot; (F) Levels of IL-6, IL-1β, and TNF-α detected by ELISA; (G) CD68 positive cells detected by immunohistochemistry. N = 6. Data were presented as mean ± standard deviation. Comparisons among multi-groups were analyzed using one-way ANOVA, followed by Tukey’s multiple comparisons test. * p < 0.05, ** p < 0.01. PVT1, plasmacytoma variant translocation 1; AS, atherosclerosis; ApoE-/-, apolipoprotein E knockout; TG, total triglyceride; TC, total cholesterol; HDL, high density lipoprotein; LDL, low density lipoprotein; MMP, matrix metalloproteinase; TIMP-1, tissue inhibitor of metalloproteinase-1; CRP, C-reactive protein; IL, interleukin; TNF-α, tumor necrosis factor-alpha.