Figure 4. Silencing PVT1 induced by siRNA inhibits the activity and proliferation of HA-VSMCs. (A) Fluorescence of GFP ensures the recombinant plasmid was transfected properly; (B) Relative expression of PVT1 detected by qRT-PCR; (C) Cell viability measured by MTT assay; (D) Cell colony formation ability detected by colony formation assay; (E) DNA replication activity detected by EdU assay. Compared to the oe-NC group, * p < 0.05, ** p < 0.01; compared to the si-NC group, # p < 0.05, ## p < 0.01. PVT1, plasmacytoma variant translocation 1; HA-VSMCs, Human arterial vascular smooth muscle cells; oe, overexpression; NC, negative control.