Research Paper Volume 13, Issue 22 pp 24640—24654

PPARα agonist relieves spinal cord injury in rats by activating Nrf2/HO-1 via the Raf-1/MEK/ERK pathway

PPARα agonist alleviated neuronal apoptosis in spinal cord of SCI rats by activating the PI3K/Akt signaling pathway. (A–C) The rats were randomly divided into sham-operation group (Sham group), rat SCI model group (SCI group), SCI + PPARα agonist PEA group (PEA group). The SCI rat model was established using modified Allen's method. The rats in the PEA group were intraperitoneally injected with PEA (2 mg/kg). Western Blotting was performed to detect apoptosis factors of Bax and Bcl-2 in spinal cord tissues; The apoptosis rate detected by TUNEL staining (scale bar = 50 μm) in spinal cord tissues; The ImageJ software was used to analyze the TUNEL-positive cells, which represented the apoptosis rate. The expression of PI3K/AKT signaling pathway-related proteins detected by Western Blotting in spinal cord tissues; ‘*’ indicates p

Figure 4. PPARα agonist alleviated neuronal apoptosis in spinal cord of SCI rats by activating the PI3K/Akt signaling pathway. (AC) The rats were randomly divided into sham-operation group (Sham group), rat SCI model group (SCI group), SCI + PPARα agonist PEA group (PEA group). The SCI rat model was established using modified Allen's method. The rats in the PEA group were intraperitoneally injected with PEA (2 mg/kg). Western Blotting was performed to detect apoptosis factors of Bax and Bcl-2 in spinal cord tissues; The apoptosis rate detected by TUNEL staining (scale bar = 50 μm) in spinal cord tissues; The ImageJ software was used to analyze the TUNEL-positive cells, which represented the apoptosis rate. The expression of PI3K/AKT signaling pathway-related proteins detected by Western Blotting in spinal cord tissues; ‘*’ indicates p < 0.05.