Research Paper Volume 13, Issue 22 pp 24640—24654

PPARα agonist relieves spinal cord injury in rats by activating Nrf2/HO-1 via the Raf-1/MEK/ERK pathway

PPARα agonist activated the Nrf2/HO-1 signaling pathway. (A–C) The rats were randomly divided into sham-operation group (Sham group), rat SCI model group (SCI group), SCI + PPARα agonist PEA group (PEA group). The SCI rat model was established using modified Allen's method. The rats in the PEA group were intraperitoneally injected with PEA (2 mg/kg). The expression of Nrf2/HO-1 signaling pathway-related proteins detected by Western Blotting in spinal cord tissues; The expression of Nrf2 detected by IHC in spinal cord tissues; Gene transcription and expression of anti-oxidative protein (HO-1 and Trx-1) detected by qRT-PCR in spinal cord tissues; ‘*’ indicates p

Figure 5. PPARα agonist activated the Nrf2/HO-1 signaling pathway. (AC) The rats were randomly divided into sham-operation group (Sham group), rat SCI model group (SCI group), SCI + PPARα agonist PEA group (PEA group). The SCI rat model was established using modified Allen's method. The rats in the PEA group were intraperitoneally injected with PEA (2 mg/kg). The expression of Nrf2/HO-1 signaling pathway-related proteins detected by Western Blotting in spinal cord tissues; The expression of Nrf2 detected by IHC in spinal cord tissues; Gene transcription and expression of anti-oxidative protein (HO-1 and Trx-1) detected by qRT-PCR in spinal cord tissues; ‘*’ indicates p < 0.05.