Research Paper Volume 13, Issue 22 pp 24740—24752

Figure 3. RACK1 is the regulatory target protein of Fam114A1. (A) In the control and sh-Fam groups, regardless of whether RACK1 or FAM1141 antibody was used for Co-IP, the mutual binding of RACK1 and Fam114A1 was observed, and it was observed that after the expression of Fam114A1 protein was inhibited, the co-precipitated RACK1 protein increased instead. (B, C) The expression of RACK1 in melanocyte of Fam, sh-Fam and their controls was detected by western bolt, the expression of RACK1 protein is negatively regulated by Fam114A1 protein. Statistical analyses were performed using one-way ANOVA and Dunnett’s post hoc test: **P < 0.01 vs Control. (D) The immunofluorescence detection of RACK1, the negatively regulation between Fam114A1 and RACK1 was confirmed once again.