Research Paper Volume 13, Issue 22 pp 24753—24767

Fisetin inhibits the proliferation, migration and invasion of pancreatic cancer by targeting PI3K/AKT/mTOR signaling


Figure 3. Fisetin induces the apoptosis of PANC-1 cells. (A) Flow cytometry evaluation of PANC-1 cells inoculated with 0, 50 and 100μM fisetin for 24 h. (B) Histogram exhibiting apoptosis rate in each group. (C) Western blot assessment of apoptosis-linked proteins. (D) Histogram illustrating cleaved caspase 3 protein contents. (E) Histogram illustrating cleaved caspase 8 protein contents. (F) Histogram illustrating cleaved PARP protein contents. All assays were replicated thrice and data are given as means±SD. *p<.05, ***p<.001, ****P<.0001, in contrast with the controls.