Research Paper Volume 13, Issue 22 pp 24753—24767

Fisetin inhibits the proliferation, migration and invasion of pancreatic cancer by targeting PI3K/AKT/mTOR signaling

Fisetin induces the apoptosis and dampens the PI3K/AKT/mTOR cascade in Patu-8988 cells. (A) Flow cytometry evaluation of Patu-8988 cells after inoculation with 0, 50 and 100μM fisetin for 24 h. (B) Histogram exhibiting apoptosis rate in each group. (C) Western blot assessment of key proteins in Patu-8988 cells inoculated with 0, 50 and 100μM fisetin for 24 h. (D) Histogram illustrating PI3K protein contents. (E) Histogram illustrating p-AKT/AKT ratio. (F) Histogram illustrating p-mTOR/mTOR ration. All assays were replicated thrice, and data are given as means±SD.*p

Figure 4. Fisetin induces the apoptosis and dampens the PI3K/AKT/mTOR cascade in Patu-8988 cells. (A) Flow cytometry evaluation of Patu-8988 cells after inoculation with 0, 50 and 100μM fisetin for 24 h. (B) Histogram exhibiting apoptosis rate in each group. (C) Western blot assessment of key proteins in Patu-8988 cells inoculated with 0, 50 and 100μM fisetin for 24 h. (D) Histogram illustrating PI3K protein contents. (E) Histogram illustrating p-AKT/AKT ratio. (F) Histogram illustrating p-mTOR/mTOR ration. All assays were replicated thrice, and data are given as means±SD.*p<.05, **p<.01, ***p<.001, ****p<.0001, in contrast with controls.