Figure 6. HDAC1 was a target gene of miR-524-5p. (A) The binding sites between miR-524-5p and HDAC1. (B) The relative luciferase activity in HEK-293T cells co-transfected with HDAC1-WT or HDAC1-Mut and with miR-524-5p mimic or NC-mimic. The mRNA levels of HDAC1 in laryngocarcinoma tissues and normal adjacent tissues (n=20) (C), laryngocarcinoma patients with different stage tumor (D) and laryngocarcinoma cell lines (E). (F) The protein expression of HDAC1 in laryngocarcinoma cell lines. (G–J) The mRNA and protein levels were detected with qPCR and Western blotting after TU212 and M2E cells transfected with si-NEAT1 or/and miR-524-5p inhibitor. * P < 0.05, ** P < 0.01 versus NC-mimic, normal adjacent tissues, laryngocarcinoma patients with stage I/II, NP69 or Scramble and NC-inhibitor.