Figure 4. Adipogenic differentiation ability of PDMSCs from different maternal age group. (A) Representative image of adipogenic differentiation of all 5 age groups. Upper panel showed that undifferentiated cells did not retain Oil-O Red staining. Lower panel showed that differentiated cells were positive to Oil-O Red staining. Real Time PCR performed to measure the gene expression of (B) Adipocyte lipid binding protein (ALBP) (C) Fatty acid-binding protein 4 (FABP4), and (D) CCAAT/enhancer-binding protein beta (CEBPB) of all 5 age groups. Group A: 18-21; Group B: 22-25; Group C: 26-30; Group D: 31-35; Group E: 36 and over. *, p<0.05; **, p<0.01; ***, p<0.0005; ****, p<0.0001. Scale bar 200 μm.