Figure 6. Circ-ZNF609 up-regulated LRRC1 expression by competitively binding miR-432-5p to induce cholangiocarcinoma development. (A) After co-transfection of circ-ZNF609 siRNA and miR-432-5p inhibitor, the LRRC1 expression was detected by qRT-PCR and western blot. (B–E) CCK-8, EdU and transwell assays confirmed that the effects of proliferation, migration and invasion induced by miR-432-5p inhibitor were partly reversed by silencing LRRC1. (F–I) Silencing circ-ZNF609 could partially reverse the proliferation and invasion induced by LRRC1 overexpression in CCK-8, EdU and transwell assays. *P< 0.05; **P< 0.01; ***P< 0.001.