Research Paper Volume 13, Issue 23 pp 25241—25255

Endothelial cell–derived exosomal circHIPK3 promotes the proliferation of vascular smooth muscle cells induced by high glucose via the miR-106a-5p/Foxo1/Vcam1 pathway


Figure 6. miR-106a-5p overexpression and Foxo1 knockdown inhibits the proliferation and promotes the apoptosis of VSMCs. (A) CCK-8 was used to detect cell viability in VSMCs overexpressing miR-106a-5p or Foxo1 (**p < 0.01 miR-106a-5p vs. mimics NC, *p < 0.05 pcDNA3.1/Foxo1 vs. pcDNA3.1). (B) Edu assay was used to detect cell viability by FCM. (C) FCM detected the apoptosis of VSMCs. (D) Western blot analysis detected the expression of Bax, Bcl2, PCNA, and Caspase 3.