Figure 6. Markers of senescence analysis in hair follicular cells. (A) p16 and p21 gene expression (mRNA level) in hair follicles from young mice (6 months old) and old mice (2.5-3 years old) in boxplot graphs of ΔCt values normalized using GAPDH or ACTB. Graphs show medians, first quartile data distribution, minimal and maximal points, and fold change values as a specifying detail. Results of a t-test are also shown (*p<0.05, **p<0.01, ***p<0.001). (B) Representative images showing the higher p16 protein levels in murine hair follicles of old animals relative to those of young animals. Images were obtained from z-stack scanning with a confocal spinning disc microscope. DAPI-stained nuclei are shown in the blue channel and p16 signals in the green channel. Autofluorescence can be seen in a hair shaft. Magnification 60x objective with oil immersion.