Research Paper Volume 13, Issue 23 pp 25325—25341

Figure 1. Art pretreatment markedly reduces UVB-irradiated cell senescence, intracellular ROS production and p16^INK4a expression and increases SOD and β-catenin expression in HaCaT cells. (A) MTT assay for determining TC50 of Art in HaCaT cells. (B, C) SA-β-gal staining for cell senescence in control group, UV group, UV + 1 μg/ml Art group, UV + 5 μg/ml Art group and UV + 20 μg/ml Art group. Scale bar: 100 μm. Magnification: 200×. (D, E) Flow cytometry of intracellular ROS production in each group. (F) Detection of SOD levels in HaCaT cells of different groups. (G–I) Western blot of the relative expression of β-catenin and p16^INK4a in each group. Ns: not significant; **p<0.01; ***p<0.001; ****p<0.0001.