Research Paper Volume 13, Issue 23 pp 25342—25364

Protective effects of galangin against H2O2/UVB-induced dermal fibroblast collagen degradation via hsa-microRNA-4535-mediated TGFβ/Smad signaling


Figure 12. Effects of galangin on the TGFβ/Smad signaling and hsa-miR-4535 expression in UVB-irradiated mice. (A and B) mRNA levels of hsa-miR-4535 and Smad4 from dorsal skin of UVB-exposed mice were detected by qRT-PCR. (C) The protein levels of TGFβ, p-smad2/3, and Smad4 in dorsal skin tissues were detected by western blotting. α-tubulin was used as a loading control. Data are presented as mean ± SD. Quantification of the results are shown; *P < 0.05, **P < 0.01 versus vehicle control group; #P < 0.05, ##P < 0.01 versus UVB plus vehicle group.