Research Paper Volume 13, Issue 23 pp 25365—25376

Anemoside B4 sensitizes human colorectal cancer to fluorouracil-based chemotherapy through src-mediated cell apoptosis


Figure 3. AB4 promotes apoptotic cell death of 5-FU resistant colorectal cancer cells. (A) Xenografts of HCT116/FU cells were implanted subcutaneously until the volume was about 100 mm3 in nude mice. The mice were treated with intravenous infusion of 5-FU (20 mg/kg) or combined with AB4 (40 mg/kg) every three days. Tumor volume was measured in the indicated day. The curves showed the mean volume ± SEM of tumor size. (B) Xenografts were harvested and imagined after 21 days treatment. The tumor volumes were compared between groups. Bar = 1 cm. (C) Representative IHC staining images of the expression of LGR5, bcl-2 and caspase 3 in the xenografts of different group. Bar, 50 μm. (D) HCT116/FU and ACC1805 cells were treated with 25 μM AB4 and 20 μM 5-FU for 48 h. Cells were collected and stained for cell apoptotic analysis by flow cytometry. (E) Statistical analysis of cell apoptosis in different groups as described in (D). The results represent three independent experiments. Data are shown as means ± SEM. *P < 0.01.