Figure 4. AB4 activates Caspase 9 pathway in 5-FU resistant colorectal cancer cells. (A) HCT116/FU and ACC1805 cells were treated with 25 μM AB4 and 20 μM 5-FU for 48 h. Control groups were treated with 5-FU only. Activity of caspase 3 was measured with pNA concentrations. (B) HCT116/FU cells were treated with 25 μM AB4 and 20 μM 5-FU. The activity of caspase-9 was measured and compared between groups in 0, 3, 6, 12, 24 hours. (C) HCT116/FU cells were treated with 20 μM 5-FU and/or 25 μM AB4. Western blot assays were performed for the Src-caspase-9 signaling pathway activation status. (D) Apoptosis related proteins (cleaved PARP, caspase 3, Bcl-2) were measured with Western blot assays. The results represent three independent experiments. Data are shown as means ± SEM. *P < 0.01.