Figure 3. The effect of TBF on AKT/NF-κB signal induced by HFD during pregnancy and lactation. 1 g/L TBF was dissolved in 0.1% carboxymethylcellulose sodium solution and was ingested by mice by drinking water. Drinking water administration began on the first day of mating and stopped one week after delivery, and then mammary tissue was collected. (A) Protein bands of p-AKT, p-P65, p-IκB, AKT, P65, IκB, β-Tublin; (B) Analysis of relative density value of p-AKT protein; (C) Analysis of relative density value of p-P65 protein; (D) Analysis of the relative density value of p-IκB protein. The data error was based on SEM, three independent repeated experiments were performed; #p < 0.01 vs. NT group; ∗∗p < 0.01 vs. HFD group.