Figure 5. The effect of TBF on the blood-milk barrier induced by HFD during pregnancy and lactation. Fresh mice mammary tissue was immersed in 2 mg/mL FITC for 15 min, then the mammary tissue block was placed in liquid nitrogen, and finally the tissue block was frozen sectioned by a cryostat. After DAPI staining, the sections were sealed with anti-fluorescence quenching reagent, finally obtain the experimental results. FITC green fluorescent protein shuttle freely between the fresh mammary acinar interstitiumed and leaked into the damaged mammary acinar. In addition, FITC will show large green fluorescence in the mammary tissue with acinar atrophy matrix thickening. The lesion was shown by the arrow in the figure, and the scale bar is 100 μm. (A) Flow trajectory of FITC solution in mammary gland; (B) Occludin protein band and relative density analysis results; (C) Analysis results of the band and relative density value of Claudin-3 protein; (D) ZO-1 protein band and relative density analysis results; β-Tublin was used as a reference for protein control. The data error was based on SEM, three independent repeated experiments were performed; #p < 0.01 vs. NT group; ∗∗p < 0.01 vs. HFD group.