Figure 7. The effect of TBF on TGF-β/Smad signal induced by HFD during pregnancy and lactation. 1 g/L TBF was dissolved in 0.1% carboxymethylcellulose sodium solution and was ingested by mice by drinking water. Drinking water administration began on the first day of mating and stopped one week after delivery, and then mammary tissue was collected. (A) Protein bands of TGF-β1, p-Smad2/3, Smad2/3, β-Tubulin; (B) Analysis of the relative density value of TGF-β1 protein; (C) Analysis of relative density value of p-Smad2/3 protein; β-Tublin was used as a reference of TBF-β1, Smad2/3 was used as a reference of p-Smad2/3. The data error was based on SEM, three independent repeated experiments were performed; #p < 0.01 vs. NT group; ∗∗p < 0.01 vs. HFD group.