Research Paper Volume 13, Issue 23 pp 25393—25407

Functional rare variant in a C/EBP beta binding site in NINJ2 gene increases the risk of coronary artery disease


Figure 2. Transcriptional factor CCAAT-box/enhancer-binding protein beta (C/EBP beta) regulates the expression of NINJ2. (A) A schematic diagram shows the predicted binding sites of C/EBP beta in the genomic region overlapping rs34166160 harbored A allele not C allele. (B) Knockdown of the expression of C/EBP beta in HUVEC resulted in the decrease of NINJ2 expression (P < 0.05). HUVECs were pretreated with scramble siRNA or C/EBP beta siRNA for 48 h, total protein extracts were prepared and blotted with the antibodies specific for NINJ2. Three independent experiments were performed. Error bars represent standard deviation (SD).